Table 3 Primers used in this study.
Construct name | Sequence (F indicates forward; R indicates reverse) |
|---|---|
5GFP-NLS1 | F: 5′-GATCCAATGGCGTCTCAAGGCACCAAACGATCATATGAACAATGCCG-3′ |
R: 5′-GATCCGGCATTTGTTCATACGATCGTTTGGTGCCTTGAGACGCCATTG-3′ | |
5GFP-NLS2 | F: 5′-GATCCAAAACGTGGAATCAATGACCGAAATTTCTGGAGGGGTGAAAATGGACGAAAGACAAGGG-3′ |
R: 5′-GATCCCCTTGTCTTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATTCCACGTTTTG-3′ | |
5GFP-NLS2 A1 | F: 5′-CTGTACAAGCAGGATCCAGCAGCTGGAATCAATGACCG-3′ |
R: 5′-CGGTCATTGATTCCAGCTGCTGGATCCTGCTTGTACAG-3′ | |
5GFP-NLS2 A2 | F: 5′-GAGGGGTGAAAATGGAGCAAAGACAGCGCCGAATC-3′ |
R: 5′-GATCCGGCGCTGTCTTTGCTCCATTTTCACCCCTC-3′ | |
5GFP-NLS2 DC | F: 5′-GATCCAAAACGTGGAATCAATGACCGAAATTTCTGGG-3′ |
R: 5′-GATCCCCAGAAATTTCGGTCATTGATTCCACGTTTTG-3′ | |
NLS1-K7A-R8A | F: 5′-GCGACCAAAGGCACCGCAGCATCTTACGAACAGATG-3′ |
R: 5′-CATCTGTTCGTAAGATGCTGCGGTGCCTTTGGTCGC-3′ | |
NLS2-R213A-R214A | F: 5′-AGGGGTGAGAATGGAGCAGCAACAAGGATTGCTTAT-3′ |
R: 5′-ATAAGCAATCCTTGTTGCTGCTCCATTCTCACCCCT-3′ |
