Table 5 Stability ranking of each candidate reference gene using different algorithms under heat shock stress treatment in shoot and root. Column “value” corresponds to M in geNorm, S in NormFinder and CV ± SD in BestKeeper.

From: Selection of reference genes suitable for normalization of qPCR data under abiotic stresses in bioenergy crop Arundo donax L.

Rank

Shoot

Root

GeNorm

NormFinder

BestKeeper

GeNorm

NormFinder

BestKeeper

Gene

Value

Gene

Value

Gene

Value

Gene

Value

Gene

Value

Gene

Value

1

TLF EF-1α

0.315

RPN6

0.133

RPN6

0,95 ± 0,24

EF-1α RPN6

0.360

RPN6

0.118

EF-1α

1,92 ± 0,39

2

TUB α

0.367

EF-1α

0.190

EF-1α

1,72 ± 0,37

TLF

0.411

EF-1α

0.131

RPN6

1,88 ± 0,46

3

RPN6

0.398

TUB α

0.282

GAPDH

2,18 ± 0,42

Act2

0.488

Act2

0.219

GAPDH

2,54 ± 0,48

4

GAPDH

0.544

TLF

0.312

TUB α

2,11 ± 0,48

GAPDH

0.542

TLF

0.239

Act2

2,28 ± 0,50

5

Act2

0.599

GAPDH

0.439

Act2

2,17 ± 0,48

TUB α

0.598

TUB α

0.292

TLF

2,48 ± 0,57

6

AC1

0.662

Act2

0.445

AC1

2,15 ± 0,51

pDUF221

0.807

GAPDH

0.352

pDUF221

2,69 ± 0,65

7

pDUF221

0.745

AC1

0.484

TLF

2,42 ± 0,56

AC1

1.019

AC1

0.849

TUB α

3,23 ± 0,76

8

  

pDUF221

0.496

pDUF221

3,56 ± 0,90

  

pDUF221

0.851

AC1

5,51 ± 1,41

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