Table 1 Nucleotide sequences of the primers used for qRT-PCR in this study.
From: D-serine, a novel uremic toxin, induces senescence in human renal tubular cells via GCN2 activation
Gene | Primer Sequence |
|---|---|
Human ß-actin | Forward 5′-TCCCCCAACTTGAGATGTATGAAG-3′ |
Reverse 5′-AACTGGTCTCAAGTCAGTGTACAGG-3′ | |
BAX | Forward 5′-TGGAGCTGCAGAGGATGATTG-3′ |
Reverse 5′-CCCAGTTGAAGTTGCCGTCAG-3′ | |
BCL-2 | Forward 5′-TGGGAGAACAGGGTACGATA-3′ |
Reverse 5′-CATCTCCCGCATCCCACTC-3′ | |
PUMA | Forward 5′-GGTCCTCAGCCCTCGCTCTC-3′ |
Reverse 5′-CTTGTCTCCGCCGCTCGTAC-3′ | |
P21 | Forward 5′-GTGGCCTTGTCGCTGTCTT-3′ |
Reverse 5′-GCGCTTGGAGTGATAGAAATCTG-3′ | |
P16 | Forward 5′-GGGGGCACCAGAGGCAGT-3′ |
Reverse 5′-GGTTGTGGCGGGGGCAGTT-3′ | |
IL-6 | Forward 5′-GGTACATCCTCGACGGCATCT-3′ |
Reverse 5′-GTGCCTCTTTGCTGCTTTCAC-3′ | |
IL-8 | Forward 5′-AAGGAAAACTGGGTGCAGAG-3′ |
Reverse 5′-ATTGCATCTGGCAACCCTAC-3′ | |
ATF4 | Forward 5′-GTTCTCCAGCGACAAGGCTA-3′ |
Reverse 5′-ATCCTCCTTGCTGTTGTTGG-3′ | |
CHOP | Forward 5′-TGCTTTCAGGTGTGGTGATGTA-3′ |
Reverse 5′-AATCAGAGCTGGAACCTGAGGA-3′ | |
GCN2 | Forward 5′-GAAGCTGTCAGCCAGCACTA-3′ |
Reverse 5′-GTTGGCAAGGGAGGTCTGAA-3′ | |
HK | Forward 5′-GGCTCATTTCCACCTCACCA-3′ |
Reverse 5′-GGAGGGCAGCATCTTAACCA-3′ | |
PFK | Forward 5′-TGAAGCCAGAGAGGCCTTAGA-3′ |
Reverse 5′- GGAACCAGGGAGAGATGTGC-3′ | |
GAPDH | Forward 5′-CCTCAACGACCACTTTGTCA-3′ |
Reverse 5′-TTACTCCTTGGAGGCCATGT-3′ | |
PGK1 | Forward 5′-CTGTGGCTTCTGGCATACCT-3′ |
Reverse 5′-GCTGCTTTCAGGACCACAGT-3′ | |
PHGDH | Forward 5′-ATCTCTCACGGGGGTTGTG-3′ |
Reverse 5′-AGGCTCGCATCAGTGTCC-3′ | |
PSAT1 | Forward 5′-CGGTCCTGGAATACAAGGTG-3′ |
Reverse 5′-AACCAAGCCCATGACGTAGA-3′ |
