Figure 3
The bone begins to form prior to vascular invasion. (A-D) A time course analysis shows regions of bone formation denoted by BSPII (red) do not overlap with the invading vasculature visualized by EMCN (green) until the bone is already in the process of being formed. Time points indicate age and time of sacrifice. P8 6 am (A), P8 1 pm (B), P9 6 am (C), P9 6 pm (D). All sections are of proximal tibia except for (D) which displays the distal femur. (E,F) COL1 (red) co-stained with EMCN (green) shows a similar developmental pattern as the BSPII time course above. P8 1 pm (E) and P8 8 pm (F). (G,H) COL1 (red) co-stained with CD31 (green) indicates that the cells visualized by EMCN are indeed the invading vasculature. P8 1 pm (G) and P9 6 am (H). Yellow boxes enlarge related regions, “ ‘ ” denotes enlarged region (e.g., A’). “A” denotes significance. Large Scale bars are 100 μm and small scale bars are 200 μm.
