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Figure 1

From: 5-methoxytryptophan protects MSCs from stress induced premature senescence by upregulating FoxO3a and mTOR

Figure 1

5-MTP protects BM-MSC from high glucose (HG) stress-induced cellular senescence. BM-MSCs were incubated in medium containing LG (5.5 mM) or HG (25 mM) for 2 weeks, chemosynthetic pure L-5-MTP (10 & 50 μM in A & B and 10 μM in C & D) was added into medium for 48 hrs before BM-MSCs were harvested and senescence markers were analyzed. (A) p21 and (B) p16 mRNA were analyzed by qPCR. (C) BrdU incorporation was analyzed as described in methods. (D) IL-6 released into the medium was measured by ELISA. Error bars in all figures denote means ± SEM (n = 3). *p < 0.05. **p < 0.01.

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