Figure 2

5-MTP suppresses HG stress-induced SA-β-gal and lysosome expansion in BM-MSCs. (A) After BM-MSCs had been cultured in LG or HG for 2 weeks they were incubated with or without 5-MTP (10 μM) for 48 hrs, washed and stained for SA-β-gal activity. SA-β-gal positive cells were quantified under light microscopy. Upper panel shows representative cell staining while lower panel quantitative analysis of SA-β-Gal positive cells. Error bars denote mean ± SEM (n = 3). *p < 0.05. **p < 0.01. (B) Lysosomes were detected by Lysotracker stain. Upper panel shows representative staining and the lower panel quantitative analyzes of cells with positive staining. Each bar denotes mean ± SEM of three experiments. *p < 0.05. **p < 0.01.