Figure 4 | Scientific Reports

Figure 4

From: 5-methoxytryptophan protects MSCs from stress induced premature senescence by upregulating FoxO3a and mTOR

Figure 4

5-MTP protects BM-MSCs from H2O2-induced senescence. (A) p21 and (B) p16 mRNA levels were analyzed by qPCR. (C) BM-MSC growth was analyzed by BrdU incorporation. (D) IL-6 released in the medium was measured with ELISA. (E) Cells with positive SA-β-Gal staining were counted and quantitatively analyzed. (F) Lysotracker staining of BM-MSCs treated with H2O2 (100 μM) in the presence or absence of 5-MTP (10 μM). Lysotracker staining was quantitatively analyzed. Error bars in all figures denote means ± SEM (n = 3). *p < 0.05. **p < 0.01.

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