Figure 6 | Scientific Reports

Figure 6

From: 5-methoxytryptophan protects MSCs from stress induced premature senescence by upregulating FoxO3a and mTOR

Figure 6

FoxO3a siRNA attenuates 5-MTP-mediated inhibition of senescence. BM-MSCs were incubated in LG-or HG- medium for 2 weeks. FoxO3a siRNA was transfected into cells for 12 h followed by 5-MTP for additional 48 h. (A) FoxO3a proteins were analyzed by Western blotting. (B) SOD and (C) catalase activity in BM-MSCs were measured under the indicated condition. (D) p16 and (E) p21 mRNA in BM-MSCs were analyzed with qPCR. (F) BM-MSCs were stained for SA-β-gal and SA-β-Gal-positive cells were counted under light microscopy. (G) IL-6 concentration in culture medium of BM-MSCs was measured with ELISA. Scramble indicated control scramble siRNA. Error bars in all figures denote means ± SEM (n = 3). *p < 0.05. **p < 0.01. N.S. non-significant.

Back to article page