Figure 7

5-MTP prevents HG-induced suppression of mTOR. BM-MSCs were cultured in LG- or HG medium for 2 weeks. 5-MTP (10 μM) was added for 48 h and cells were lysed and (A) mTOR proteins and (B) p70S6 kinase were analyzed by western blotting. Upper panel shows representative blots and the lower panel the densitometric analysis. Error bars denote mean ± SEM (n = 3). *p < 0.05. **p < 0.01.