Figure 8
Rapamycin abrogates 5-MTP-mediated protection against senescence. BM-MSCs were incubated in LG-or HG- medium for 2 weeks. Rapamycin (5 μM) was added for 24 h followed by 5-MTP for additional 48 h. (A) p16 and (B) p21 mRNA in BM-MSCs were analyzed with qPCR. (C) BM-MSCs were stained for SA-β-gal and SA-β-Gal-positive cells were counted under light microscopy. (D) IL-6 concentration in culture medium of BM-MSCs was measured with ELISA. Error bars in all figures denote means ± SEM (n = 3). *p < 0.05. **p < 0.01. N.S. non-significant.
