Table 4 Results of global analysis of fluorescence decay kinetics on isolated proteins.

From: Functional modulation of LHCSR1 protein from Physcomitrella patens by zeaxanthin binding and low pH

Sample

A1

τ1 (ps)

A2

Τ2 (ps)

τavg (ps)

LHCSR1 CTRL pH 7.5 0.03% a-DM

26%

974

74%

3756

3042

LHCSR1 DEP pH 7.5 0.03% a-DM

39%

750

61%

3123

2198

LHCSR1 CTRL pH 5 0.03% a-DM

44%

634

56%

4080

2567

LHCSR1 DEP pH 5 0.03% a-DM

52%

551

48%

2971

1720

LHCSR1 CTRL pH 7.5 0.007% a-DM

52%

198

48%

3446

1742

LHCSR1 DEP pH 7.5 0.007% a-DM

49%

366

51%

2982

1687

LHCSR1 CTRL pH 5 0.007% a-DM

81%

87

19%

1574

372

LHCSR1 DEP pH 5 0.007% a-DM

82%

86

18%

677

192

LHCSR1 CTRL pH 7.5 0.003% a-DM

69%

109

31%

1559

553

LHCSR1 DEP pH 7.5 0.003% a-DM

64%

110

36%

1175

495

LHCSR1 CTRL pH 5 0.003% a-DM

64%

67

36%

235

127

LHCSR1 DEP pH 5 0.003% a-DM

100%

81

81

  1. 2D Streak camera maps were fitted with a bi-exponential decay function with a global analysis method. Amplitude and time constants are reported. Average fluorescence lifetimes were calculated as ΣAiτi/ΣAi.