Figure 3
From: Astrocytes and oligodendrocytes in grey and white matter regions of the brain metabolize fatty acids
Alkyne lipids localize to reporter-expressing oligodendrocytes and astrocytes. (a,b) Brain slices of transgenic mice expressing the red-fluorescent tdTomato-reporter after tamoxifen-induced recombination in (a,b) oligodendrocytes (PLP-CreERT2), or (c,d) astrocytes (GLAST-CreERT2) were incubated with 50 µM of (a,c) alkyne palmitate (aPal), or (b,d) alkyne oleate (aOle) for 2 h. Fluorescence microscopy after click-reaction was performed showing alkyne lipids (green) and fluorescent protein (red) in merged channel overview micrographs. Inserts depict close-up images of individual cells with co-localizing signals. All images were recorded with equal settings and are shown at equal intensity levels. Scale bars, 50 μm.
