Figure 3 | Scientific Reports

Figure 3

From: Ligand activation induces different conformational changes in CXCR3 receptor isoforms as evidenced by plasmon waveguide resonance (PWR)

Figure 3

Conformational change in response to antagonist. (A) Changes in the minimum resonance position following incremental addition of CXCR3 agonist PS372424, or CXCR3 antagonist SCH546738 to HEK-CTRL, HEK-CXCR3-A and HEK-CXCR3-B cell fragments as a function of time obtained with p-(left panel) and s-(right panel) polarized lights. (B) PWR shifts obtained with p-(left panel) and s-(right panel) polarized lights for HEK-CXCR3-A and HEK-CXCR3-B after treatment of SCH546738 (1 h at 2.2 nM). (C) KD values for PS372424 or SCH546738 interaction with CXCR3-A in HEK-CXCR3-A and HEK-CXCR3-B cells. All the results represent three independent experiments combined to calculate mean and SEM, and values were normalized to those obtained for the control cells.

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