Figure 4 | Scientific Reports

Figure 4

From: Ligand activation induces different conformational changes in CXCR3 receptor isoforms as evidenced by plasmon waveguide resonance (PWR)

Figure 4

Calcium-flux and PWR shifts in the presence of agonist and antagonist. (A) Relative calcium-induced flux in HEK-CXCR3-A and HEK-CXCR3-B cells after 1 h of SCH546738 pre-treatment at concentration from 0 to 2.2 nM. SCH546738 concentration inhibiting 50% of the relative calcium-induced flux were indicated as EC50 values. (B) PWR shifts obtained with p-(left panel) and s-(right panel) polarized lights for HEK-CXCR3-A (left panel) and HEK-CXCR3-B (right panel) after treatment of SCH546738 alone (1 h at 1 nM) or after addition of PS372424 after SCH546738 at 1 nM. (C) Binding curve and KD values for PS372424 interaction with CXCR3-B before and after SCH546738 treatment at 1 nM. All the results represent three independent experiments combined to calculate mean and SEM, and values were normalized to those obtained for the control cells.

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