Figure 1
From: Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
Residual mesenchyme envelops pancreatic epithelium in scaffold-free, three-dimensional organotypic culture. (a) Schematic procedure for the generation of organotypic pancreatoids. Dissection of the dorsal pancreatic bud (dorsal panc. bud) at embryonic day 10.5 (E10.5) precedes removal of mesenchyme by dispase and dissociation of cells before seeding in organogenesis media. Scale bar = 50 um. (b) Brightfield images of Matrigel-embedded organoids with mesenchyme removed and scaffold-free, free-floating organotypic pancreatoids. Scale bar = 50 um. (c) Whole mount three-dimensional reconstruction of Matrigel-embedded organoid and scaffold-free organotypic pancreatoid stained by immunofluorescence, with the epithelium marked by Pdx1 (in green) and mesenchyme marked by Vimentin (in red). Scale bar = 50 um.
