Table 1 qPCR primers.
From: Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
Gene | f (5′–3′) | r (5′–3′) |
|---|---|---|
Gapdh | AATGTGTCCGTCGTGGATCTGA | AGTGTAGCCCAAGATGCCCTTC |
Nkx6.1 | GACCTGACTCTCCGTCATCC | TTGTTGGACAAAGATGGGAAG |
Pax6 | TCTAATCGAAGGGCCAAATG | AGGAGGAGACAGGTGTGGTG |
Hnf6 | TTCTTGCTCTTTCCGTTTGC | CCCTGGAGCAAACTCAAGTC |
Ins1 | GGAGCGTGGCTTCTTCTACA | CTGCAGCACTGATCCACAAT |
Ins2 | TCTACAATGCCACCACGCTTCTG | TTTGTCAAGCAGCACCTTTG |
Prss1 | GTGGTAGCCAGAGTTCAGGG | ACTCCTGTTCCTGGCCCTT |
Isl1 | GCATTTGATCCCGTACAACC | CTGAGGGTTTCTCCGGATTT |
Mist1 | TCATAGCTCCAGGCTGGTTT | AAGCTACGTGTCCTTGTCCC |
Pdx1 | CCACCCCAGTTTACAAGCTC | CGTGAGCTTTGGTGGATTTC |
Hes1 | CCAAGCTAGAGAAGGCAGACA | TGATCTGGGTCATGCAGTTG |
