Figure 2 | Scientific Reports

Figure 2

From: Identification of a p53 target, CD137L, that mediates growth suppression and immune response of osteosarcoma cells

Figure 2

Identification of CD137L, CDC42BPG and FST as p53 direct target genes. (a–c) Luciferase assay of the p53BS in human (left) or mouse (right) CD137L (a), CDC42BPG (b) or FST (c) using SaOS2 cells. Luciferase activity is indicated relative to the activity of the mock vector with SD (n = 3). *P < 0.05, *P < 0.001, Student’s t-test. (d–f) CHIP assay for CD137L (d), CDC42BPG (BS-C) (e) or FST (f) was performed using SaOS2 cells that were infected with Ad-LacZ (lane 1) or Ad-p53 (lane 2-4). DNA-protein complexes were immunoprecipitated with an anti-p53 antibody (lanes 1 and 2) followed by qPCR. Input chromatin represents a small portion (1%) of the sonicated chromatin collected prior to immunoprecipitation. Immunoprecipitates with an anti-IgG antibody (lane 3) or in the absence of an antibody (lane 4) were used as negative controls. Columns, mean; error bars, SD (n = 3).

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