Figure 4 | Scientific Reports

Figure 4

From: Structural basis for functional selectivity and ligand recognition revealed by crystal structures of human secreted phospholipase A2 group IIE

Figure 4

Phospholipid head group selectivity of hGIIE. (a–c) Enzymatic activity of hGIIE determined by isothermal titration calorimetry method: (a) 1, 2-dihexanoyl-sn-glycero-3- phosphor-ethanolamine (DHPE); (b) 1, 2-dihexanoyl-sn-glycero-3-phosphor-rac-1-glycerol (DHPG); (c) 1, 2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC). Heat flows are converted to specific enzyme activities. The raw titration curves are shown in the inserts. Data is fitted to the standard Michaelis-Menten equation, trend line is shown in red. (d–f) Docking model of hGIIE with the phospholipid: (d) hGIIE-DHPE; (e) hGIIE-DHPG; (f) hGIIE-DHPC. Hydrogen bond interaction is indicated by black dash lines, and residues involved are shown as stick.

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