Figure 7
The effect of Arf6 ablation from LECs on tumor progression. (A) Scheme of the assay for the effect of Arf6 ablation from mLECs on tumor progression. Tamoxifen (3 mg) was daily injected into the peritoneal cavity of the mice for one week, and B16 melanoma cells (2 × 104 cells) were subcutaneously transplanted into the right lower back region of the mice. The size of the tumor was measured by digital caliper every two days from day 6 after the transplantation. (B) Tumor volumes produced in vehicle-treated Arf6 flox/flox, tamoxifen-treated Arf6 flox/flox, and tamoxifen-treated LEC-Arf6 cKO mice were measured according to the schedule described in (A). (C) At 14 days of the transplantation, tumors were dissected, and 3 examples were shown. The data shown in (B) are mean ± SEM from three independent experiments. Statistical significance was assessed using One-way ANOVA. NS, not significant. **P < 0.01.
