Figure 6
From: Transglutaminase 2 regulates osteoclast differentiation via a Blimp1-dependent pathway
TG2 deficiency decreases trabecular bone mass. (a,b) Three-dimensional micro-CT images of the distal femurs of 13-week-old WT and TG2 KO mice (n = 6 for each group) were reconstructed with Skyscan CTvol software. The sagittal and transaxial images generated with DataViewer software are shown. Scale bar, 500 μm. (c) The trabecular bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) in distal femurs were quantified by micro-CT analysis with Skyscan CTAn software. (d) Decalcified sections of distal femurs of WT and TG2 KO mice were stained for TRAP activity or with hematoxylin/eosin (H&E). Scale bar, 100 μm. (e) The number of osteoclasts per bone perimeter (N.Oc/B.Pm), osteoclast surface per bone surface (Oc.S/BS), eroded surface per bone surface (ES/BS), and the number of osteoblasts per bone perimeter (N.Ob/B.Pm) were quantified. *P < 0.05 versus WT mice. NS, not significant.
