Figure 4

MTP8 OE plants are more tolerant to high levels of Mn. (a) Wild type (WT) and MTP8 OE plants grown on B5 (59 μM Mn) with or without supplementation of 2 mM Mn for 17 days. (b) Chlorophyll levels and shoot fresh weights of WT, and MTP8 OE plants in (a). Each biological replicate is a pool of 5 plants. Error bars represent SE (n = 3). Asterisks indicate P < 0.05 by t test. (c,d) Mn and Fe concentrations of shoot (c) and root (d) of wild type (WT) and MTP8 OE plants grown on B5 with or without supplementation of 1.5 mM Mn. Error bars represent SE (n = 5). Asterisks indicate P < 0.05 by t test.