Figure 1
From: STIM1-dependent Ca2+ signaling regulates podosome formation to facilitate cancer cell invasion
Cancer tissues overexpress STIM1 and SOCE. (a) Representative images of immunofluorescent staining for STIM1 expression in surgical specimens of cervical cancer. Nuclei were stained with Hoechst 33258 (blue). Scale bars, 50 μm. (b) The stacked column chart illustrating the intensity grade for STIM1 expression in surgical specimens of cervical cancer (n = 27). ***P < 0.001 by Chi-square test. (c) Representative intracellular Ca2+ ([Ca2+]i) measurement in primary tissue cultures derived from paired cancer and adjacent non-cancerous tissues (Case #1). Each trace is the mean [Ca2+]i measurement of at least 30 cells. The SOCE amplitude indicates the rise of [Ca2+]i in the replenishment of extracellular calcium ([Ca2+]o) from 0 to 2 mM. Arrow, adding 2 μM thapsigargin (TG). (d) Quantitative analyses of SOCE at the primary tissue cultures from 3 cervical patients with paired cancer and adjacent non-cancerous tissues. Each value represents mean ± S.E.M. of at least 30 cells. *P < 0.05, **P < 0.01, by unpaired t test.
