Figure 2
From: STIM1-dependent Ca2+ signaling regulates podosome formation to facilitate cancer cell invasion
STIM1 is involved in the formation of invadopodia. Cells were transiently transfected with two STIM1-specific siRNA prior to being seeded on the gelatin-coating coverslips. (a) Knockdown efficiency of siControl, siSTIM1 in breast cancer MDA-MB-231 cells. Cropped blots have been presented. Full length blots are presented in Supplementary Fig. S4 (b) Representative confocal images showing the expression of F-actin and cortactin in breast cancer MDA-MB-231 cells. F-actin (red), cortactin (green), Scale bar, 20 μm. Arrow, invadopodia. (c) Quantitative analyses of cells with invadopodia at siControl and siSTIM1 groups in breast cancer MDA-MB-231 cells. Values represent mean ± S.E.M. from three independent experiments. **P < 0.01, ***P < 0.001, compared with control groups. (d) Knockdown efficiency of siControl, siSTIM1 in osteosarcoma U2OS cells. Cropped blots have been presented. Full length blots are presented in Supplementary Fig. S5 (e) Representative confocal images showing the expression of F-actin and cortactin in osteosarcoma U2OS cells. F-actin (red), cortactin (green), Scale bar, 20 μm. Arrow, invadopodia. (f) Quantitative analyses of cells with invadopodia at siControl and siSTIM1 groups in osteosarcoma U2OS cells. Values represent mean ± S.E.M. from three independent experiments. **P < 0.01, ***P < 0.001, compared with control groups.
