Figure 3

Attenuation of SOCE activity decreases the podosome rosette formation in v-Src-transformed MEFs. (a) Left, Representative intracellular Ca²⁺ ([Ca²⁺]_i) measurement in v-Src-transformed MEFs. Each trace is the mean [Ca²⁺]_i measurement of at least 100 cells. The SOCE amplitude indicates the rise of [Ca²⁺]_i in the replenishment of [Ca²⁺]_o from 0 to 2 mM. First arrow, adding 2 μM thapsigargin (TG). Second arrow, adding 0.1% DMSO or SKF-96365 (20 or 50 μM). Right, Quantitative analyses of SOCE activity. Each value represents mean ± S.E.M. of at least 100 cells. *P < 0.01 **P < 0.001 by unpaired t test. (b) Left, representative intracellular Ca²⁺ ([Ca²⁺]_i) measurement in v-Src-transformed MEFs. Each trace is the mean [Ca²⁺]_i measurement of at least 100 cells. The SOCE amplitude indicates the rise of [Ca²⁺]_i in the replenishment of [Ca²⁺]_o from 0 to 2 mM. First arrow, adding 2 μM thapsigargin (TG). Second arrow, adding 0.1% DMSO or 2-APB (20 or 50 μM). Right, quantitative analyses of SOCE activity. Each value represents mean ± S.E.M. of at least 100 cells. *P < 0.01 **P < 0.001 by unpaired t test. (c) The v-Src-transformed MEFs were pre-incubated with 0.1% DMSO or SKF-96365 (20 and 50 μM) and 2-APB (20 and 50 μM) for 24 hours. The cells were stained for F-actin. Scale bar, 30 μm. (d) Quantitative analyses of the cells with podosome rosettes. Values represent mean ± S.E.M. from at least 200 individual cells. **P < 0.01, ***P < 0.001, compared with control groups.