Figure 5
Using of SPMF for Ag-Ab interaction detection of BGH in mouse: (a) response of the cantilever when 4 nM anti-BGH is injected into the SPMF. Three phases of biosensing includes: (i) before injection of Ag; (ii) during the stress induction; and (iii) the final response. A mean steady-state deflection of δtip = 35 µm was observed within 90 sec from the end of Ag injection; (b) mean steady-state deflection of the cantilever tip due to different antigen concentration and calculation of detection limit. A limit of detection of 2 ng/ml (100 pm) was observed with a minimum deflection resolution of 1 µm; (c) the results of the presented SPMF showing a big improvement in the analysis time and limit of detection of current state-of-the-art biosensors. Further improvement of cantilever readout system can promote the detection limit of SMPF even into untouched femto-molar and even atto-molar region [Adapted from J. Arlett et al.1].
