Figure 5 | Scientific Reports

Figure 5

From: Factor Xa Mediates Calcium Flux in Endothelial Cells and is Potentiated by Igg From Patients With Lupus and/or Antiphospholipid Syndrome

Figure 5

Affinity purification of anti-FXa IgG and effects on FXa-mediated intracellular signaling. The anti-(a)FXa sub-fraction was affinity purified from n = 3 patients with the hightest FXa binding. (A) Gel showing flowthrough of serum passed through the Heparin column (lane 2), wash-step (lane 3), purification through aFXa column (lane 4) and subsequent IgG purification by Sepharose protein G (lane 5). (B) A graph showing the aFXa activity of IgG fractions: column 1, flow-through of heparin column; column 2, fraction containing IgG non-specifically bound to heparin; and column 3, affinity purified aFXa IgG. The values are OD at 450 nm. (C) Representative (n = 1) western blot and (D) densitometric analysis (n = 3) showing that FXa reactive whole IgG and affinity purified aFXa IgG significantly (p < 0.05) upregulates phosphorylation of NFkB in comparison to FXa alone or IgG alone.

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