Figure 1

The expression of CYGB is associated with the cell survival ability against oxidative stress in hCPCs. (a) Representative images and quantitative data of Western blot showed that the expression of CYGB is observed in both hCPCs and human right atrial appendage (hRAA). However, the expression of myoglobin is not detectable in hCPCs compared to its abundant expression in hRAA. The total protein loading is 5 µg for hRAA and 20 µg for hCPCs. Full-length blots are presented in Supplemental Fig. S1. (b) Western blot confirmed that CYGB was overexpressed in hCPCs. Full-length blots are presented in Supplemental Fig. S1. (c) CPCs were infected with lentivirus expressing CYGB or vector for 24 h, challenged with 2 mM H₂O₂ for 3 h, and then evaluated by LDH assay. (d) Western blot confirmed that CYGB was knocked-down in hCPCs with shRNAs for clone #1&4. Full-length blots are presented in Supplemental Fig. S8. (e) Human CPCs were infected with lentivirus expressing shRNA against CYGB, or scramble shRNA for 24 h, challenged with 2 mM H₂O₂ for 3 h, then evaluated by LDH assay. Data presented in this figure were mean values on a ratio of H₂O₂-induced LDH release to total LDH in cells with standard error (means ± SEM). *Indicates p < 0.05 vs. control; **indicates p < 0.01 vs. control; n = 4 independent experiments.