Figure 2 | Scientific Reports

Figure 2

From: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

Figure 2

Overexpression of CYGB exhibits anti-apoptotic effect. (a) Human CPCs were infected with CYGB- or vector-lentivirus for 24 h, and challenged with 1 mM H2O2 for 1.5 h, then evaluated by Annexin/PI FACS assay with quantitative analysis. (b) Representative images for TUNEL staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 24 h, and challenged with 2 mM H2O2 for 2.5 h. (c) Examination of anti-apoptotic gene expression at the mRNA level after CYGB was overexpressed. (d) Examination of pro-apoptotic gene expression at the mRNA level after CYGB was overexpressed. (e) Representative images and quantitative data of Western blot showing anti-apoptotic and pro-apoptotic protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. S10. *Indicates p < 0.05 vs. control; **indicates p < 0.01 vs. control; n = 3 independent experiments.

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