Figure 2 | Scientific Reports

Figure 2

From: Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

Figure 2

Effect of the deletion of the GacS detection domain on rsm, T3SS and T6SS gene expression. (a) Activities of the rsmZ–lacZ (left) and rsmY–lacZ (right) transcriptional chromosomal fusions were monitored at different growth stages in the PAK WT (open triangle), PAKΔgacS (open square) or PAKgacSΔPD (open circle) strain. The corresponding β-galactosidase activities are expressed in Miller units as the mean values (with error bars) of three independent experiments. (b) Transcript levels of VgrG1b (T6SS; dark blue bar) and ExoS (T3SS; light blue bar) were monitored in the PAK, PAK∆gacS and PAKgacSΔPD strains using RT-qPCR; fold change is displayed for the two mutant strains compared to PAK strain. *, ** and *** refer to p < 0.05, p < 0.01 and p < 0.001, respectively, according to the moderated t-tests.

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