Figure 7
Effect of mutations in the GacS detection domain on biofilm formation. (a) Biofilm production in glass tubes (upper panel) is illustrated and quantified after crystal violet staining (lower panel). The corresponding levels of biofilm production represent means values (with error bars) obtained from three independent experiments. *, **, *** and ns referred to p < 0.05, p < 0.01 and p < 0.001 and non-significant difference, respectively, according to the Wilcoxon-Mann-Whitney tests. (b) Biofilm formation monitored by confocal laser scanning microscopy after 12 h. Extracted z images and their respective xy and xz planes are shown.
