Figure 8
Effect of mutations in the GacS detection domain on rsm genes, T3SS and T6SS expression. (a) Activities of the rsmZ–lacZ (left panel) and rsmY–lacZ (right panel) transcriptional chromosomal fusions were monitored at different growth stages in the PAKWT, PAKΔgacS, PAKgacS R94A , PAKgacS H97A , PAKgacS H124A PAKgacS H133A strains. The corresponding β-galactosidase activities are expressed in Miller units and correspond to mean values (with error bars) obtained from three independent experiments. (b) Transcript levels of VgrG1b (T6SS; black bar) and ExoS (T3SS; white bar) were monitored in the PAK, PAKΔgacS, PAKgacS R94A , PAKgacS H97A , PAKgacS H124A and PAKgacS H133A strains using RT-qPCR and fold change is displayed for the four mutant strains compared to the PAK strain. *, ** and *** refer to p < 0.05, p < 0.01 and p < 0.001, respectively, according to the moderated t-tests.
