Figure 4
From: Flavonoid allosteric modulation of mutated visual rhodopsin associated with retinitis pigmentosa
Chemical stability of the immunopurified WT and G90V mutant. Immunopurified protein samples in PBS pH 7.4 and 0.05% DM, after Q treatment, were incubated with 50 mM hydroxylamine, pH 7 and the decrease of Amax was recorded over time at 20 °C. (A) WT 11CR with (⚪) and W/O 1 µM Q (●). (B) WT 9CR with (⚪) and W/O 1 µM Q (●). (C) G90V 11CR with (⚪) and W/O 1 µM Q (●). (D) G90V 9CR with (⚪) and W/O 1 µM Q (●). Mean value and standard error (SE) obtained in independent purifications (n = 3).
