Figure 4 | Scientific Reports

Figure 4

From: Chromatin remodeling system p300-HDAC2-Sin3A is involved in Arginine Starvation-Induced HIF-1α Degradation at the ASS1 promoter for ASS1 Derepression

Figure 4

Induction of PHD2 and HDAC2 interaction by ADI. (a) Induction of HDAC2 and PHD2 interaction by ADI determined by co-IP using anti-HDAC2 antibody followed by Western blotting using anti-PHD2 and anti-HDAC2 antibodies. (b) Reciprocal co-IP using anti-PHD2 in immunoprecipitation and Western blots using anti-PHD2 and anti-HDAC2. (c) Co-IP in 293 cells transfected with recombinants encoding HDAC2-Flag and PHD2-GFP. (d,e) ChIP assays of ASS1 promoter bindings of PHD2 and HDAC2 in PHD2-knockdowned A2058 cells with siRNA (A and B). (f) Effect of HDAC2 knockdown on ASS1 promoter bindings of PHD2 and HDAC2.

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