Figure 3
From: Cholesterol-modified Hydroxychloroquine-loaded Nanocarriers in Bleomycin-induced Pulmonary Fibrosis
Histological examinations of the anti-fibrotic effects and the time kinetic of Chol-HCQ liposomes in bleomycin-induced pulmonary fibrosis. Rats were treated with bleomycin and then injected with Chol-HCQ liposomes (20 mg/kg/day) and HCQ liposomes (8 mg/kg/day) via the tail vein; PBS solution and null liposomes (PC) were used as controls. (a) On day 28, the rats were sacrificed and histological examination was performed by H&E staining (up) and Masson’s trichrome staining (below). Original magnification is 200x (n = 6); the data are representative of three separate experiments. (b) Bleomycin-induced rats were treated with Chol-HCQ liposomes or PBS as a control. On days 7, 14 and 28, the rats were sacrificed and histological examination was performed by H&E staining (n = 4). (c) The hydroxyproline contents in the lung tissues were examined on day 28 (n = 6). (d) Lung lavages were collected and examined by differential cell counting in the BALF on day 28 of the experiment; the total cell numbers, neutrophils, macrophages, eosinophils and lymphocytes were counted. Original magnification is 200x; data are representative of three separate experiments. *p < 0.05.
