Figure 1

Only the direct CAP treatment can trigger the H₂O₂ production of specific cancer cell lines in vitro. (a) The H₂O₂ concentration in DMEM after the CAP treatment on just DMEM (control), on pancreatic adenocarcinoma cells (PA-TU-8988T) immerged in DMEM, on breast adenocarcinoma cells (MDA-MB-231) immerged in DMEM, as well as on glioblastoma cells (U87MG) immerged in DMEM. (b) The cells-based H₂O₂ concentration. The data is calculated based on the following formula. Cells-based H₂O₂ concentration = H₂O₂ concentration in the DMEM which has been used to immerse cancer cells during the CAP treatment – H₂O₂ concentration in the CAP-treated DMEM (control). The H₂O₂ concentration corresponds to the mean of each bar shown in 1a. (c) The indirect CAP treatment will not stimulate cancer cells to generate H₂O₂. All H₂O₂ concentration was measured immediately (about 1 min) after the treatment. Results are presented as the mean ± s.d. of three independently repeated experiments. Student’s t-test was performed between the data based on cells immersed in specific volume of DMEM and the data just based on the DMEM with the same volume. The significance is indicated as *p < 0.05.