Figure 2
From: The Strong Cell-based Hydrogen Peroxide Generation Triggered by Cold Atmospheric Plasma

H2O2 evolution in the DMEM surrounding cancer cells after the direct CAP treatment. (a) Cancer cells immersed in 20 μL of DMEM. (b) Cancer cells immersed in 100 μL of DMEM. CAP was used to treat DMEM (control) or cancer cells (MDA-MB-231 cells, PA-TU-8988T cells, and U87MG cells) immersed in DMEM. In each experiment, the 1 min of CAP treatment was performed on the cells immersed in 20 μL or 100 μL of DMEM. The H2O2 concentration was measured very minute after the CAP treatment until the tenth minute. Cancer cells in 96-wells plate were cultured overnight before the CAP treatment. The cell confluence was 3 × 104 cells/mL. Results are presented as the mean ± s.d. of three independently repeated experiments.