Figure 3
From: The Strong Cell-based Hydrogen Peroxide Generation Triggered by Cold Atmospheric Plasma
The cell confluence and the output voltage affect the cell-based H2O2 generation. (a) A relative small confluence can achieve the maximum cell-based H2O2 generation. (b) High output AV voltage significantly weakens the cell-based H2O2 generation. In each experiment, a 1 min of CAP treatment was used to treat DMEM (control) or cancer cells (MDA-MB-231 cells, PA-TU-8988T cells, and U87MG cells) immersed in 100 μL of DMEM. The H2O2 concentration was measured immediately (about 1 min) after the treatment. Cancer cells were cultured overnight before the treatment. The cell confluence for (b) was 3 × 104 cells/mL. Results are presented as the mean ± s.d. of three independently repeated experiments. Student’s t-test was performed and the significance is indicated as *p < 0.05.
