Figure 4
From: The Strong Cell-based Hydrogen Peroxide Generation Triggered by Cold Atmospheric Plasma

Under the same experimental conditions, the anti-cancer capacity of the direct CAP treatment is significantly stronger than the indirect CAP treatment. For the indirect CAP treatment, the CAP-stimulated DMEM was used to affect the growth of cancer cells. Comparison between the direct CAP treatment and the indirect treatment is performed on (a) MDA-MB-231 cells and (b) PA-TU-8988T cells. Pre-treating cancer cells with 6 mM NAC an intracellular scavenger for 3 hr or immediately (about 1 min) renewing the DMEM after the direct CAP treatment can effectively weakens the anti-cancer capacity of the direct CAP treatment on (c) MDA-MB-231 cells and (d) PA-TU-8988T cells. Results are presented as the mean ± s.d. of three independently repeated experiments. Student’s t-test was performed between the results from the direct CAP treatment and the results from the indirect CAP treatment. The significance is indicated as *p < 0.05.