Table 1 Mixed size micro-particle experiments.

From: Real-time Image Processing for Microscopy-based Label-free Imaging Flow Cytometry in a Microfluidic Chip

Experiments

Human counting

R-MOD counting

 

Duration

Count (mean ± s.d.)

Count (7/10/15-μm) Throughput

Number ratio (est. 100/34/10)

Exp-1 (6 μL/min)

4 sec

2,343 ± 6.3

2,346 (1,540/549/257) 587 cells/s

100/35/10

Exp-2 (10 μL/min)

4 sec

—

3,103 (2,423/462/218) 776 cells/s

100/19/9

Exp-3 (18 μL/min)

4 sec

—

5,362 (3,055/1,306/1001) 1,340 cells/s

100/43/33

Exp-Large (18 μL/min)

17 min

—

635,844 (396,122/133,891/105,831) 616 cells/s

100/34/27

  1. From Exp-1 to Exp-3, 2,000 recorded images (during 4 sec) were processed by R-MOD. To quantitative evaluation of counting results, six human participants counted flowing beads by viewing the recorded image sequence of Exp-1. Error of counting result between human and R-MOD is 3. Since beads in the suspension are not uniformly distributed, every experiment shows different actual throughput (from 600–1,300 cells/s). Exp-Large analysed a large number of images (>510,000) during 17 minutes to show the high-throughput and real-time capability of the proposed system. Number ratio is calculated based on the number concentration of the mixed bead to roughly compare the actual detected counts.
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