Figure 2 | Scientific Reports

Figure 2

From: Acetyl-4′-phosphopantetheine is stable in serum and prevents phenotypes induced by pantothenate kinase deficiency

Figure 2

Synthesis, characteristics and rescue potential of P-PantSAc. (a) Schematic synthesis route of P-PantSAc. (b) Stability of P-PantSAc in foetal calf serum at 37 °C was determined using HPLC analysis and compared to the known compounds PantSH (non-serum-stable control) and P-PantSH (stable-serum control). Stability in serum for each compound is given as % of stability in PBS. For stability measurement in PBS n = 2 samples were measured. (c-e) Toxicity of PantSH, P-PantSH and P-PantsAc, respectively, was tested in HEK293 cells by determining cell numbers after 4 days of incubation with the compounds using increasing concentrations. (f-h) Rescue potential of P-PantSAc was tested in cells with impaired PANK activity using HoPan in S2 cells (f) or in HEK293 cells (g) or in cells treated with dPANK/fbl RNAi (h), as a control an RNAi construct was used specific for a irrelevant human gene hMAZ. (For all graphs mean ± SD is given. Two-tailed Student t-test was performed. *p < 0.05, **p < 0.01, ***p < 0.001).

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