Figure 3
From: A Novel H2S-releasing Amino-Bisphosphonate which combines bone anti-catabolic and anabolic functions
DM-22 inhibits h-OCs differentiation and function without inducing cytotoxicity and preserving a residual h-OCs population. (A) Histogram showing LDH measurements. Data are expressed as percent cytotoxicity and refers to arbitrary units obtained by colorimetric detection of LDH activity. Data are expressed as mean ± SD of triplicates of N = 3 independent experiments. One sample t-test was performed for statistical analyses (*p < 0.05, **p < 0.01, ***p < 0.001 DM-22 vs AL). (B) Representative pictures of TRAP staining (magnification 20X), showing the effect of DM-22 and AL on h-OCs differentiation. (C) Histograms showing the average number of TRAP+ h-OCs per microscope field. At least 8 fields were counted for each well in duplicate. Data are expressed as mean ± SD of N = 3 independent experiments. Mann-Whitney test (####p < 0.0001 DM-22 vs AL) or Wilcokon signed rank test (*p < 0.05, **p < 0.01, ****p < 0.0001 vs CTRL = 100%) were performed for statystical analyses. (D) Representative pictures of pit assay (magnification 20X), showing the effect of DM-22 and AL on h-OCs function.
