Figure 4
From: A Novel H2S-releasing Amino-Bisphosphonate which combines bone anti-catabolic and anabolic functions
DM-22 is devoid of AL-like cytotoxicity on h-MSCs. (A) Representative pictures of toluidin blue staining showing the morphology of AL or DM-22 treated h-MSCs (33 μM) compared to untreated cells (CTRL) and cells treated with Triton X-100 (CTRL+). Magnification 20x. (B) Histogram showing toluidin blue staining quantification (absorbance 560 nm). Data are expressed as mean ± SD of N = 3 independent experiments (each one in quadruplicate). One way Anova and Tukey’s multiple comparison test were performed for statistical analyses. (C,D) Histograms showing LDH measurements in h-MSCs treated with DM-22 or AL compared to CTRL cells at 24 h (C) and 72 h (D); data are expressed as percent cytotoxicity and refers to arbitrary units obtained by colorimetric detection of LDH activity. Data are expressed as mean ± SD of N = 3 independent experiments (each one in quadruplicate). Mann Whitney test was performed for statistical analyses (*p < 0.05, ****p < 0.0001 DM-22 vs AL). (E,F) Histograms showing h-MSCs proliferation as revealed by 3H-thymidine detection for DM-22 and AL compared to CTRL cells at 24 h (E) and 72 h (F) Data are expressed as fold increase compared to CTRL sample and are expressed as mean ±SD of N = 4 independent experiments (each one in quadruplicate). Wilcoxon signed rank test (*p < 0.05, **p < 0.01) and Mann-Whitney test (#p = 0.0516) were performed for statistical analyses.
