Figure 6
Non-α-granule proteins with reducing levels during storage. (a) Non-imputed LFQ values and concentrations measured employing ELISA of S100A9. (b) Non-imputed LFQ values of S100A8. (c) Non-imputed LFQ values and Western blot analysis of SEMA4D including a representative Western blot analysis of SEMA4D under reducing and non-reducing conditions. (d) Non-imputed LFQ values and Western blot analysis of A2M including a representative Western blot of A2M. Calmodulin was used as loading control. Band normalization was performed as in Fig. 4. Data represents mean ± standard deviation (n = 3), in (d) values of all replicates are shown for LFQ values. *P < 0.05 compared to day 1 from this time point onwards. LFQ values of 0 indicates that protein was not detected. Uncropped immunoblots of SEMA4D, A2M and calmodulin can be found in Supplementary Data S5.
