Figure 1
From: A Toxic Conformer of Aβ42 with a Turn at 22–23 is a Novel Therapeutic Target for Alzheimer’s Disease
The protective effect of 24B3 on the neurotoxicity induced by Aβ42. (a) A schematic illustration of the structure of the toxic conformer of Aβ42, which possesses a turn structure at 22–23 (“toxic turn”). The toxic turn formation draws Y10 and M35 closer to accelerate radical transition31, which contributes to the formation of the hydrophobic core in the C-terminus of Aβ4229,30, resulting in the stable assembly as low-molecular-weight oligomers32,43. 24B3 was developed as a conformation-specific antibody against the toxic turn structure. The epitopes of the conventional anti-Aβ antibodies, 6E10 and 82E1, are E3-S8 and N-terminus, respectively. (b and c) The antibodies are mixed with Wt-Aβ42 (b) or E22P-Aβ42 (c) before the treatment, and the mixtures were applied to primary cortical neurons. Wt-Aβ42 and E22P-Aβ42 were applied at a concentration of 1 µM for 96 h. Antibodies were applied at a concentration of 0.1 mg/ml (approximately 0.685 µM). ***p < 0.001 vs vehicle (Veh). #p < 0.05, ###p < 0.001.
