Figure 2
From: Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome
Numbers of OTUs (Chao1 index) and 3D-PCoA based on un-weighted UniFrac distances. Amplicons of 16S rDNA were sequenced using 27Fmod and 338R primers. (a) Sequences were clustered into OTUs with a 97% identity threshold and the α-diversity index (Chao1) was calculated for each sample. In Stage III, Chao1 was significantly lower than in the other groups. (b) Multidimensional composition of each group was determined based on matrix data for un-weighted UniFrac distance. Clustering of Stage III (red) samples differed from that of Stage 0-I (blue)/Normal AF (green)/Blank (grey); Stage II (yellow) was scattered between the two clusters. Three-D PCoA was performed with R; *P < 0.05, **P < 0.01, ***P < 0.001 by Mann–Whitney test.
