Figure 3

Methylation at cytosines in different contexts by DNMT3a and DNMT3b in mouse (a,c) and human (b) ESCs. (a) The window-slided (window size = 10 bp) average CpH methylation levels around mCpG (methylation level >0.8) in WT, DNMT1-KO, and DNMT3-KO mouse ESC. (b) Bar graphs showing the average methylation levels at CpHpH (blue) and CpHpG (red) contexts in wild type (WT), DNMT3a-knock out (D3a-KO), DNMT3b-knock out (D3b-KO), and DNMT3a/b-knock out (D3-DKO) HUES64 cell line. WT ESC is HUES64.2. (c) Average methylation level at CAC, CAT, CAA, and CAG contexts in mouse ESCs, in which DNMT1, DNMT3a, and DNMT3b were knocked out (TKO), and DNMT3a and DNMT3b were reintroduced (TKO+3a and TKO+3b), respectively²⁴. Red and grey bars respresent average mCpH level in mCpG-proximal regions (mCpG level>0.5) and whole genome, respectively.