Figure 3

Transfection of miRNA mimic and inhibitor into HepaRG cells and its effect on the protein levels in APAP treated cells (N = 3). Western blot analysis of miR-122 mimic and inhibitor transfected cells treated with APAP for 12 h. (A) CYP1A2 and (B) CYP3A4: Ctrl = Control untreated; APAP = 20 mM; miR-122 Mimic 5 nM; miR-122 Inhibitor 100 nM. Uncropped image of blots for CYP1A2 and CYP3A4 are shown in Figure S3. Western blot analysis of miR-378 mimic and inhibitor transfected cells (C) CYP2E1: Ctrl = Control untreated; APAP = 20 mM; miR-378a Mimic 5 nM; miR-378a Inhibitor 50 nM. Uncropped image of blot for CYP2E1 shown in Figure S4. Data are normalized to β-Actin (*p < 0.05, **p < 0.01) and error bars represent standard error of the mean (SEM). (D) ALT levels in miR-122 and miR-378a mimic and inhibitor transfected cells treated with 20 mM APAP for 12 h. “h” denotes hour.