Figure 1 | Scientific Reports

Figure 1

From: Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris

Figure 1

Time course of the three-phase fermentation strategy for the production of VAMAX1 (straight lines) and VAMAX2 (dotted lines) at the 15-L scale. The strategy included a typical glycerol fed-batch phase to increase the cell density before induction. An oxygen-limited induction mode with a constant methanol concentration of 2.5 mL L−1 was used for product expression. (a) Course of dry cell weight (DCW, •) and dissolved oxygen tension (DOT). (b) Course of added glycerol (Gly) and methanol (Me), methanol concentration (MC), and product concentration (▴). The x-axis is normalized to the time point of induction. Error bars indicate the standard deviation of technical triplicates.

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