Figure 4 | Scientific Reports

Figure 4

From: Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris

Figure 4

Analysis of fermentation supernatants during the two-phase fermentation process for the production of VAMAX1. (a) Proteins in 15 µL supernatant were separated by LDS-PAGE and stained with SimplyBlue SafeStain. (b) The proteins separated above were further analyzed by immunoblotting using the reduction-sensitive mAb 4G2. Times above the lanes indicate the elapsed hours of each process phase. M: protein marker. EoB: end of batch-phase. V1: target protein VAMAX1.

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