Figure 1 | Scientific Reports

Figure 1

From: In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins

Figure 1

Live cell STED microscopy of neurons expressing F-actin labels fused to mNeptune2. Neurons were transduced with (a) Lifeact-mNeptune2, and (b) Actin-Chromobody-mNeptune2, at 11 days in vitro. Living neurons were measured with an excitation wavelength of 586 nm shown as the conventional confocal image (left) and with an additional STED laser at 775 nm resulting in the superresolution STED image (right). Inset of (a): Line profiles, taken across the indicated filament (green arrows); average of three lines of raw data with Lorentz fit (STED) or Gaussian fit (Confocal) and full-width at half-maximum (FWHM). In STED-mode the FWHM measured at 4 positions (85 nm green arrow, 86 nm, 87 nm, and 74 nm, white arrows) averages to 83 nm.

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